Apr 23, · Phorbol ester treatment of MCF-7 cells led to the tyrosine phosphorylation and activation of PKC δ. However, through Western blot analysis and in . To examine the role of PKC in the progression of breast cancer, human MCF-7 breast cancer cells were transfected with PKC-alpha, and a group of heterogenous cells stably overexpressing PKC-alpha were isolated (MCFPKC-alpha). MCFPKC-alpha cells expressed fivefold higher levels of PKC-alpha as compared to parental or vector-transfected MCF.
The involvement of novel protein kinase C isozymes in influencing sensitivity of breast cancer MCF-7 cells to tumor necrosis factoralpha. By Alakananda Basu. Abstract. Protein kinase C (PKC) has been implicated in tumor necrosis factor-a (TNF) signaling. Structurally and functionally distinct PKC activators and selective inhibitors of PKC were. Burkitt Lymphoma cells,11 immature thymocytes,12 salivary gland acinar cells,13 human MCF-7 breast cancer cells and LNCaP cells.1,15 Using genetic and pharmacologic inhibition of specific PKC isozymes Garcia-Bermejo et al.2 showed that both PKCα and PKCδ mediate TPA-induced apoptosis in LNCaP cells, although the.
Protein kinase C (PKC) modulates growth, differentiation and apoptosis in a cell-specific fashion. Overexpression of PKC-alpha in MCF-7 breast cancer cells (MCFPKC-alpha cell) leads to expression of a more transformed phenotype. The response of MCF-7 and MCFPKC-alpha cells to phorbol esters (TPA) was examined. Aug 01, · A. BasuThe involvement of novel protein kinase C isozymes in influencing sensitivity of breast cancer MCF-7 cells to tumor necrosis factor-alpha Mol. Pharmacol., 53 (), pp. CrossRef Google Scholar.
There is an inverse relationship between ER status and protein kinase C alpha (PKCa) in breast cancer (Borner et al. ), breast cancer cell lines (Tonetti et al. ), and endometrial cancer. Dec 25, · A marked synergistic induction of AP activity was observed when the cells were incubated with both agents simultaneously. Two AP isozymes, tissue‐nonspecific (TNAP) and intestinal (IAP), were shown to be expressed in MCF‐7 cells as confirmed by the differential rate of thermal inactivation of these isozymes and RT‐PCR.